Microwave-stimulated antigen retrieval is pH and temperature dependent.
نویسندگان
چکیده
منابع مشابه
High temperature antigen retrieval and loss of nuclear morphology: a comparison of microwave and autoclave techniques.
The use of high temperature antigen retrieval methods has been of major importance in increasing the diagnostic utility of immunocytochemistry. However, these techniques are not without their problems and in this report attention is drawn to a loss of nuclear morphological detail, including mitotic figures, following microwave antigen retrieval. This was not seen with an equivalent autoclave te...
متن کاملThe Comparison Between Microwave and Autoclave as Antigen Retrieval Methods for Immunohistochemical Detection of CD15 and CD30 in Hodgkin’s Lymphoma
Background and Objective: Hodgkin's lymphoma is a potentially curable hematologic malignancy with difficulty in its diagnosis especially in atypical cases even in expert hands. Today, immunohistochemistry plays a significant role in the diagnosis of it especially applying the anti-CD15 and anti-CD30 antibodies. The negativity of CD15 can be reduced by antigen retrieval for meth...
متن کاملDetection of Bromodeoxyuridine in Paraffin- embedded Tissue Sections Using Microwave Antigen Retrieval is Dependent on the Mode of Tissue Fixation
bstract A simple routine microwave antigen retrieval procedure allows the sensitive detection of incorporated BrdU in pulse labeled cells. Of the two fixatives tested, Carnoy’s offers superior nuclear morphology, but with a sacrifice of immunostaining intensity. For investigations where animals are sacrificed within several hours after pulse labeling, Carnoy‘s fixative may prove adequate as a g...
متن کاملInfluence of temperature and pH on heterogenetic bacterial antigen.
A heterogenetic antigen, labile to heat (100 C) at alkaline pH, was found in many species of gram-positive bacteria (Rantz et al., J. Infectious Diseases 98:211, 1956). Erythrocytes sensitized with this antigen are agglutinated by homologous antibodies in normal human serum with heterogenetic antibodies in bacterial antisera from man and rabbit. Staphylococcus aureus, Listeria mo'nocytogenes, a...
متن کاملIba1 immunoreactivity is enhanced following an antigen retrieval treatment with EDTA, pH 6.0
Antigen retrieval is a standard procedure to enhance immunohistochemical detection. However, among the many choices of techniques available for antigen retrieval, it is important to choose a method that works specifically for the antibody of interest. The small calcium binding protein, Iba1, has been well characterized as a microglia specific marker useful for identifying both resting and activ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1994
ISSN: 0022-1554,1551-5044
DOI: 10.1177/42.12.7983356